The aim of this study was to investigate the period of estrus cycle in aceh cattle, Indonesia, based on vaginal cytology techniques.\nFour healthy females of aceh cattle with average weight of 250ââ?¬â??300 kg, age of 5ââ?¬â??7 years, and body condition score of 3-4 were\nused. All cattle were subjected to ultrasonography analysis for the occurrence of corpus luteum before being synchronized using\nintramuscular injections of PGF2 alpha 25 mg. A vaginal swab was collected from aceh cattle, stained with Giemsa 10%, and\nobserved microscopically. Period of estrus cycle was predicted from day 1 to day 24 after estrus synchronization was confirmed\nusing ultrasonography analysis at the same day. The result showed that parabasal, intermediary, and superficial epithelium were\nfound in the vaginal swabs collected from proestrus, metestrus, and diestrus aceh cattle. Proportions of these cells in the particular\nperiod of estrus cycle were 36.22, 32.62, and 31.16 (proestrus); 21.33, 32.58, and 46.09 (estrus); 40.75, 37.58, and 21.67 (metestrus);\nand 41.07, 37.38, and 21.67 (diestrus), respectively. In conclusion, dominant proportion of superficial cell that occurred in estrus\nperiod might be used as the base for determining optimal time for insemination
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